Journal: Biology direct

Article Title: RGS5 maintaining vascular homeostasis is altered by the tumor microenvironment.

doi: 10.1186/s13062-023-00437-y

Figure Lengend Snippet: Fig. 5 High RGS5 expression is associated with increased tumor vasculature in the patients with breast cancer. (A) Representative immunohistochemical staining for RGS5, α-SMA and CD34 expression in TNBC and adjacent normal tissues. Scale bar = 100 μm. Right: Quantification of the immunohistochemistry (n = 50). (B) Western blot and quantification of α-SMA and RGS5 in TNBC tissues. (C) Representative immunofluorescence images of RGS5 and α-SMA in TNBC tissues. Scale bar = 100 μm. The data were presented as the mean ± SD

Article Snippet: After blocking with 5% milk in TBST, the membranes were incubated with primary antibodies against VCAM-1 (1:1000, Epitomics), RGS5 (1:500, Proteintech), MCP-1(1:200, Santa Cruz), C3 (1:250, Abcam), CD74 (1:500, Abcam), Lyz2 (1:200, Proteintech) α-SMA(1:1000, Proteintech), Caspase-1 (1:200, Proteintech), GSDMD (1:200, Abcam) or β-actin (1:1000, Proteintech) at 4°C overnight, and then with the HRPconjugated secondary antibody for 1 h. The blots were visualized using Image Quant LAS 4000 detection system (GE Healthcare).

Techniques: Expressing, Immunohistochemical staining, Staining, Immunohistochemistry, Western Blot, Immunofluorescence

Journal: Oncology Letters

Article Title: Upregulating MMP-1 in carcinoma-associated fibroblasts reduces the efficacy of Taxotere on breast cancer synergized by Collagen IV

doi: 10.3892/ol.2018.9092

Figure Lengend Snippet: Characterization of primary cultured CAFs. The cultured cells were flat spindle shaped, rich in cytoplasm and flat ovoid nuclear in morphology. Immunostaining revealed that the primary cultured CAFs demonstrated positive expression of α-SMA and Vimentin, with negative expression of CK (magnification, ×100). CAF, carcinoma-associated fibroblast; α-SMA, α-smooth muscle actin; CK, multi-cytokeratin.

Article Snippet: Immunohistochemistry (IHC) IHC staining for α-smooth muscle actin (α-SMA), multi-cytokeratin (CK) and Vimentin (all purchased from ZSGB-Bio, Beijing, China) was performed.

Techniques: Cell Culture, Immunostaining, Expressing